西双版纳州健康人群血清丙氨酸氨基转移酶参考区间调查

目的建立西双版纳州健康人群血清丙氨酸氨基转移酶(ALT)丙酮酸氧化酶法参考区间。方法对3167名健康者血清ALT活性在不同性别、不同年龄组间的分布进行统计分析。结果ALT活性男性明显高于女性(P<0.001)。ALT参考区间上限:男性为42 U/L,女性为37 U/L。男性血清ALT活性在30~59岁组较高(其中40~49岁组最高),<20岁组最低;ALT参考区间上限:<20岁为34 U/L,20~29岁为46 U/L,30~59岁为47 U/L,≥60岁为39 U/L。女性≥40岁者血清ALT活性较高(其中50~59岁组最高),≤29岁者较低;ALT参考区间上限:≤29岁为34 U/L,30~39岁为35 U/L,≥40岁为40 U/L。结论ALT活性在不同性别、不同年龄组间有明显差异,各实验室应按不同性别、不同年龄建立本地区、本实验室血清ALT参考区间。     

Parathyroid surgery and methylene blue: A review with guidelines for safe intraoperative use

Methylene blue has been used to help facilitate parathyroid surgery for over 30 years. Its use has been widely considered both safe and cost effective. Twenty‐six cases of a toxic metabolic encephalopathy, however, have been reported with its use. As a result, some surgeons have stopped using this technique altogether. It is now known that methylene blue is a monoamine oxidase inhibitor. When combined with drugs that increase central serotonin neurotransmission, serotonin toxicity results. This is the cause of the encephalopathy described in the literature. A case report, review of the literature, and guidelines as to its proper use are presented so as to allow for safe parathyroid surgery. Laryngoscope, 2009     

单胺氧化酶基因多态性与注意缺陷多动障碍智商的相关性研究

目的探讨单胺氧化酶基因30bp VNTR(MAOA uVNTR)多态性对中国汉族注意缺陷多动障碍(ADHD)患儿智商的影响。方法对1999年9月至2003年10月在北京大学精神卫生研究所门诊就诊、符合美国《精神障碍诊断与统计手册(4版)》(DSM IV)诊断标准的333例中国汉族ADHD患儿使用中国 韦氏儿童智力量表评定智商,并检测MAOA uVNTR多态性。按照基因型将样本分为低活性基因型组和高活性基因型组,比较两组间智商(包括总智商、言语智商、操作智商)差异,并对影响智力的相关因素进行多元线性回归分析。结果单因素分析结果显示,影响ADHD患儿智商的因素有MAOA uVNTR多态性和父亲文化程度,其中低活性基因型组患儿总智商、言语智商和操作智商得分明显高于高活性基因型组,差异均有统计学意义(P值分别为0.006、0.011和0.042)。本科及以上文化程度父母其子女智商最高,高中(中专大专)次之,初中及以下者最低。父亲文化程度对患儿总智商、言语智商及操作智商等的影响差异均有统计学意义(P值分别为0.015、0.048和0.040);母亲文化程度对患儿总智商影响差异有统计学意义(P=0.33)。经多因素分析,只有MAOA uVNTR基因型进入回归方程,对患儿的总智商产生影响(P=0.021)。结论MAOA uVNTR多态性与ADHD患儿智商相关。     

Capsaicin exerts therapeutic effects by targeting tNOX-SIRT1 axis and augmenting ROS-dependent autophagy in melanoma cancer cells

Although considered a sporadic type of skin cancer, malignant melanoma has regularly increased internationally and is a major cause of cancer-associated death worldwide. The treatment options for malignant melanoma are very limited. Accumulating data suggest that the natural compound, capsaicin, exhibits preferential anticancer properties to act as a nutraceutical agent. Here, we explored the underlying molecular events involved in the inhibitory effect of capsaicin on melanoma growth. The cellular thermal shift assay (CETSA), isothermal dose-response fingerprint curves (ITDRF_CETSA), and CETSA-pulse proteolysis were utilized to confirm the direct binding of capsaicin with the tumor-associated NADH oxidase, tNOX (ENOX2) in melanoma cells. We also assessed the cellular impact of capsaicin-targeting of tNOX on A375 cells by flow cytometry and protein analysis. The essential role of tNOX in tumor- and melanoma-growth limiting abilities of capsaicin was evaluated in C57BL/6 mice. Our data show that capsaicin directly engaged with cellular tNOX to inhibit its enzymatic activity and enhance protein degradation capacity. The inhibition of tNOX by capsaicin was accompanied by the attenuation of SIRT1, a NAD⁺-dependent deacetylase. The suppression of tNOX and SIRT1 then enhanced ULK1 acetylation and induced ROS-dependent autophagy in melanoma cells. Capsaicin treatment of mice implanted with melanoma cancer cells suppressed tumor growth by down-regulating tNOX and SIRT1, which was also seen in an in vivo xenograft study with tNOX-depleted melanoma cells. Taken together, our findings suggest that tNOX expression is important for the growth of melanoma cancer cells both in vitro and in vivo, and that inhibition of the tNOX-SIRT1 axis contributes to inducting ROS-dependent autophagy in melanoma cells.     

广西两地理株钉螺COⅠ基因分析及其与邻近四省钉螺亲缘关系的比较

目的 了解广西两地理株钉螺细胞色素氧化酶Ⅰ(cytochrome oxidase Ⅰ,CO Ⅰ)基因的差异性,探讨广西钉螺与周边邻近4省钉螺的亲缘关系.方法 采集广西靖西有肋、横县光壳钉螺,提取DNA,PCR扩增CO Ⅰ基因并测序.登录GenBank检索出云南大理、四川绵竹、湖南岳阳、湖北汉阳4地钉螺的CO Ⅰ基因序列...     

Interaction between chitosan and natamycin maintain freshness quality and enzymatic activity of post-harvest Prunus salicina Lindl. cv. ‘Shazikongxinli’ fruit

A novel natural method was developed to preserve the freshness quality of Prunus salicina Lindl. cv. ‘Shazikongxinli’ fruit during post-harvest storage. The fresh-keeping paper soaked in different solutions containing chitosan and natamycin was developed and applied to the ‘Shazikongxinli’ fruit for physiochemical, biochemical, and enzymatic activities. Chitosan-natamycin interaction provided an excellent protective effect on ‘Shazikongxinli’ fruit quality during post-harvest storage compared to other treatments. This result could be confirmed by the lowest browning index, polyphenol oxidase activity, and weight loss percentage. Besides, vitamin C, peroxidase, and titratable acid were recorded highest in the chitosan-natamycin treated group, which is 0.32 mg 100 g^?1, 6.0 U min g^?1, and 4.50 mol L^?1 at 15 days, respectively. Furthermore, interlinkages between phenolic content, polyphenol oxidase (PPO) activity, and browning index were also recorded, which played an influential role in maintaining fruit quality. The study results could have commercial applications for preserving qualities and extending the shelf-life of fruits.     

糖皮质激素受体功能对大鼠前额叶单胺氧化酶A的影响及其交互作用*

目的：通过原代培养神经元细胞，探究糖皮质激素受体（GR）功能对单胺氧化酶A （MAO A）的影响及 其可能通路。方法： 取生后48 h 内的SD大鼠前额叶，经反复消化沉淀后，对神经元进行纯化并培养，48 h 后， 分为地塞米松（DEX）组、米非司酮（mifepristone，RU486）组和对照组，分别予以GR激动剂DEX、GR抑制 剂RU486 和等体积相同溶剂干预，继续培养48 h。采用免疫细胞化学染色法检测大鼠神经细胞Sp1、Krüppel 样 转录因子-11（KLF11）和MAO A表达情况。结果： DEX 干预后，前额叶神经细胞中KLF11、Sp1 及MAO A的 表达均较对照组升高，而RU486 干预后，Sp1 和MAO A的表达较对照组降低，但KLF11 的表达无明显变化。结论： 糖皮质激素（GC）可提高前额叶区GR的表达；GC可通过激活GR，提高脑内神经细胞KLF11、Sp1 和MAO A 表达水平，Sp1 的表达和MAO A存在正相关，提示GR功能与MAO A之间可能通过KLF11、Sp1 起交互作用。